All sizes of total RNA can be purified, from large mRNA and rRNA to small RNA <200 nt, such as viroids, miRNA, and siRNA
All total RNA is isolated from the plant or fungi sample, which may include possible pathogens or endosymbiotic organisms in plants—an excellent tool for plant pathology studies
Rapid and convenient nucleic acid isolation and purification process is completed in 30 min. without the use of liquid nitrogen for homogenization of samples
Simple procedure does not require additional steps such as salt precipitations and ultracentrifugations
Optimized RNA purification protocol thoroughly extracts the full complement of RNA from cells and tissues containing complex polysaccharides and polyphenolic substances which are detrimental in other RNA isolation procedures
Protocol requires no hazardous organic extractions such as phenol and chloroform
Purified RNA is of the highest quality and can be used for downstream applications analyzing gene expression, including QPCR, QRT-PCR, primer extension, gene expression array assays, and Northern blotting
Specifications
QPCR, QRT-PCR, primer extension, gene expression array assays and northern blotting