Biopolymer Separations

 HPLC Columns and Chromatographic Media for the Analysis and Purification of Biopolymers

Biopolymers, including peptides, proteins, polynucleotides, and other biodegradable polymers, vary in terms of net ionic charge, hydrophobicity, size, shape and mass. In addition to these structural (and functional) complexities, an individual biopolymer is typically present at low concentration in samples that contain many similar and similarly complex compounds. Thus, peptides, proteins, and polynucleotides are often analyzed, isolated and purified by multiple modes of chromatography, including size-exclusion, ion-exchange, reversed phase, hydrophobic interaction, hydrophilic interaction, and/or affinity chromatography.

Sigma-Aldrich/Supelco offers a wide variety of stationary phases for the analysis and purification of large biomolecules, developed and manufactured by Supelco or in cooperation with our technology partners. In addition, Sigma-Aldrich/Supelco offers columns from well-known brands, including Tosoh Bioscience, GE Life Sciences, Sepax Technologies and Kromasil.

Our portfolio of U/HPLC and FPLC columns for biopolymer separations is organized by mode of chromatography, and by column brand or manufacturer. Recent additions to the product portfolio include BIOshell™ Glycan columns to determine the glycan composition of glycoproteins, gel-filtration and ion-exchange columns from Sepax^® Technologies, and the complete line of TSKgel^® HPLC columns and Toyopearl^® media and process development columns from Tosoh Bioscience.

Bookmark this page as we continue to add new applications, technical papers and exciting separation tools to advance your biopolymer research.

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